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KMID : 1137020170280040004
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Journal of Gynecologic Oncology
2017 Volume.28 No. 4 p.4 ~ p.4
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Genotype-specific methylation of HPV in cervical intraepithelial neoplasia
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Hsu Yaw Wen
Huang Rui Lan
Su Po Hsuan
Chen Yu Chih
Wang Hui Chen
Liao Chi Chun
Lai Hung Cheng
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Abstract
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Objective: Hypermethylation of human papillomavirus (HPV) and host genes has been reported in cervical cancer. However, the degree of methylation of different HPV types relative to the severity of the cervical lesions remains controversial. Studies of the degree of methylation associated with the host gene and the HPV genome to the severity of cervical lesions are rare. We examined the association of methylation status between host genes and late gene 1 (L1) regions of HPV16, 18, 52, and 58 in cervical brushings.
Methods: Cervical brushings from 147 HPV-infected patients were obtained. The samples comprised normal (n=28), cervical intraepithelial neoplasia (CIN) 1 (n=45), CIN2 (n=13), and CIN3/carcinoma in situ (n=61). The methylation status of HPV and host genes was measured using bisulfite pyrosequencing and quantitative methylation-specific polymerase chain reaction (PCR).
Results: The degree of methylation of L1 in HPV16, 18, and 52 was associated with the severity of the cervical lesion. In HPV52, C-phosphate-G (CpG) sites 6368m, 6405m, and 6443m showed significantly higher methylation in lesions ≥CIN3 (p=0.005, 0.003, and 0.026, respectively). Methylation of most HPV types except HPV52 (r
Conclusion: Our study showed that the degree of L1 methylation of HPV16, 18, and 52 but not 58 is associated with the severity of cervical lesions. The association between HPV methylation and host gene methylation suggests different responses of host cellular epigenetic machinery to different HPV genotypes.
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KEYWORD
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DNA Methylation, Viruses, Methylation, PAX1 Transcription Factor, Cervical Intraepithelial Neoplasia, Human Papillomavirus 16, Human Papilloma Virus
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